马铃薯根际促生菌的筛选、鉴定的研究
马铃薯根际促生菌的筛选、鉴定的研究(任务书,开题报告,论文9500字)
摘要
植物根际促生菌是一类有益于植物生长的微生物，它不仅可以给植物提供必要的营养元素，如氮钾磷肥，还可以拮抗病毒侵害，抑制重金属的影响。本研究是从马铃薯根际土壤中分离并筛选出来植物根际促生菌，利用16sDNA基因序列分析方法多次从细菌和真菌方面确认筛选出来的菌株是植物根际促生菌，后续对三株筛选出来的菌株进行测序，得出AZC-5菌株属于农杆菌属（Agrobacterium spp.）、FTG-1菌株属于糖化芽孢杆菌（Paenibacillus glycanilyticus strain）、ZKC-24菌株属于假单胞菌属(Pseudomonas spp.)。
通过钼锑蓝比色法测出又具有优异的溶磷能力，其中AZC-5菌株溶磷量为37.89mg/L，ZKC-24菌株溶磷量为42.38mg/L，FTG-1菌株溶磷量为35.54mg/L。除此之外，还鉴定了菌株的生长素IAA的分泌量，AZC-5菌株分泌IAA值最高，为77.66mg/L。其他两株稍弱于此，FTG-1菌株为31.03mg/L，ZKC-24菌株为44.46mg/L。
关键词：植物根际促生菌；16sDNA；钼锑蓝比色法；溶磷量；分泌生长素
Abstract
Plant rhizosphere bacteria is a kind of microorganism which is beneficial to plant growth. It can not only provide essential nutrients for plants, such as n potassium phosphate fertilizer, but also antagonize the virus invasion and inhibit the influence of heavy metals. At present, in order to screen out the excellent growth promoting bacteria from the rhizosphere of various plants, this study is to isolate and screen the rhizosphere bacteria from the rhizosphere soil of the potato. The strains screened from bacteria and fungi by the 16sDNA sequence analysis method are the rhizosphere bacteria of plant rhizosphere, and the following three strains are screened out. The strain was sequenced and the AZC-5 strain belonged to Agrobacterium tumefaciens and FTG-1 strains belonging to Paenibacillus glycanilyticus strain and ZKC-24 strain belonging to the genus Pseudomonas (Pseudomonas spp.). [版权所有：http://DOC163.com]
It has excellent phosphate solubilization ability by molybdenum antimony blue colorimetric method, in which the amount of phosphorus soluble in AZC-5 strain is 37.89mg/L, the amount of phosphorus soluble in ZKC-24 strain is 42.38mg/L, and the amount of phosphorus soluble in FTG-1 strain is 35.54mg/L. In addition, the secretion of auxin IAA was identified. The highest IAA secretion of AZC-5 strain was 77.66mg/L. The other two strains were slightly weaker, the FTG-1 strain was 31.03mg/L, and the ZKC-24 strain was 44.46mg/L.
Key words: Plant Rhizosphere growth promoting bacteria; 16sDNA; molybdenum antimony blue colorimetry; phosphorus solubilizing capacity; secretion of auxin.
[资料来源：http://doc163.com]
目录
第一章 文献综述 1
1.1植物与微生物的关系 1
1.1.1微生物与植物概述 1
1.1.2根际土壤微生物 1
1.2植物根际促生菌的研究背景和意义 1
1.3植物根际促生菌的研究进展 2
1.3.1植物根际促生菌的定义 2
1.3.2植物促生菌的多样性 2
1.3.3植物促生菌的作用机理 3
1.3.3促生菌促进植物生长的生物学特性 3
1.3.4植物促生菌的基本应用 4 [来源：http://www.doc163.com]
第二章 马铃薯根际固氮菌的筛选、鉴定 6
2.1实验材料 6
2.1.1筛选培养基： 6
2.1.2分离纯化培养基： 7
2.1.3比色液 7
2.1.4PCR所用的引物 8
2.2试验方法 8
2.2.1配制盐溶液（mg/L） 8
2.2.2处理土壤 8
2.2.3接种 8
2.2.4纯化 8
2.3促生菌的鉴定 9
2.3.1菌落形态观察 9
2.3.2分泌植物生长素测定 9
2.3.3促生菌株16sRNA水平鉴定 10
2.3.4溶磷性测试 11
第三章 结果与分析 13
3.1促生菌筛选 13
3.2纯化后菌株16sRNA基因PCR扩增分析 13
3.3菌落形态观察 13
3.3.1革兰氏染色结果 13
3.3.2芽孢染色结果 14
3.3.3菌落形态记录 14 [资料来源：http://www.doc163.com]
3.4分泌生长素测定结果 15
3.4.1制作IAA标准曲线 15
3.4.2 PGPR菌株分泌IAA的能力 16
3.5植物根际PGPR菌株溶磷能力测定 16
3.5.1磷标准曲线的绘制 16
3.5.2三株菌株溶磷能力 17
第四章 展望 18
参考文献 19 [来源：http://www.doc163.com]